Journal: Nutrients

Article Title: S -Allylmercaptocysteine Attenuates Cisplatin-Induced Nephrotoxicity through Suppression of Apoptosis, Oxidative Stress, and Inflammation

doi: 10.3390/nu9020166

Figure Lengend Snippet: S-allylmercaptocysteine (SAMC) attenuated cisplatin-induced cytotoxicity in human kidney (HK-2) cells. Cell viability was assessed using the SRB (sulphorhodamine B) assay. HK-2 cells were treated with various concentrations of cisplatin ( A ), SAMC ( B ), Cells were pretreated with or without SAMC (50–100 μM) for 4 h and then cultured in the presence or absence of 8 μg/mL cisplatin for 24 h ( C , D ) HK-2 cells treated with 8 μg/mL cisplatin and 8 μg/mL cisplatin plus 100 μM SAMC were stained with DAPI (4’6-diamidino-2-phenylindole) to investigate apoptotic-like nuclear morphology. White arrows indicate condensed nuclei. All data were obtained from three independent experiments and presented as the means ± SEM (standard error of the mean). Bars with different characters are statistically different at * p < 0.05 level.

Article Snippet: DAPI (4′6-diamidino-2-phenylindole) dye kit was obtained from Beijing Solarbio Co., Ltd. (Beijing, China).

Techniques: Cell Culture, Staining